A sodium/proton antiporter in chromaffin-granule membranes
نویسندگان
چکیده
منابع مشابه
Interaction of calmodulin with adrenal chromaffin granule membranes.
We have reported that calmodulin bound specifically to catecholamine-containing secretory granules of adrenal medullary chromaftin cells [I]. Binding was dependent upon pM levels of Ca2+ and saturated at a low level (3.3. pmol calmodulin/mg granule membrane protein). Additionally, we observed that the phosphorylation of membrane polypeptides of M, 59 000, 58 000 and 53 000 was stimulated by cal...
متن کاملPurification and properties of a vanadate- and N-ethylmaleimide-sensitive ATPase from chromaffin granule membranes.
A vanadate- and N-ethylmaleimide-sensitive ATPase was purified about 500-fold from chromaffin granule membranes. The purified preparation contained a single major polypeptide with an apparent molecular mass of about 115 kDa, which was copurified with the ATPase activity. Immunological studies revealed that this polypeptide has no relation to subunit I (115 kDa) of the H+-ATPase from chromaffin ...
متن کاملBovine Chromaffin Granule Membranes Undergo Ca"-regulated Exocytosis in Frog Oocytes
We have devised a new method that permits the investigation of exogenous secretory vesicle function using frog oocytes and bovine chrornaffin granules, the secretory vesicles from adrenal chromaffin cells. Highly purified chrornaffin granule membranes were injected into Xenopus laevis oocytes . Exocytosis was detected by the appearance of dopamineß-hydroxylase of the chrornaffin granule membran...
متن کاملBovine chromaffin granule membranes undergo Ca(2+)-regulated exocytosis in frog oocytes
We have devised a new method that permits the investigation of exogenous secretory vesicle function using frog oocytes and bovine chromaffin granules, the secretory vesicles from adrenal chromaffin cells. Highly purified chromaffin granule membranes were injected into Xenopus laevis oocytes. Exocytosis was detected by the appearance of dopamine-beta-hydroxylase of the chromaffin granule membran...
متن کاملIsolation of ATPase I, the proton pump of chromaffin-granule membranes.
Chromaffin-granule membranes contain two ATPases, which can be separated by (NH4)2SO4 fractionation after solubilization with detergents, or by phase segregation in Triton X-114. ATPase I (Mr 400000) is inhibited by trialkyltin, quercetin and alkylating agents, and hydrolyses both ATP and ITP. It contains up to five types of subunit, including a low-Mr hydrophobic polypeptide that reacts with d...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1989
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2570499